Journal: Molecular Vision

Article Title: Radiation cataracts: mechanisms involved in their long delayed occurrence but then rapid progression

doi:

Figure Lengend Snippet: The presence of free DNA and ROS in the x-ray cataract. Using LSCM with fluorescent dyes for DNA and ROS, integrated sequential sections from the surface down to 120 μm beneath the lens surface revealed the following in a 3.5+ x-ray cataract that developed 5 months post-radiation: A : Free and particulate DNA is present in the left upper part of the irradiated lens. B : A control animal at the same age and time is free of the abnormal internal DNA content. C : A great amount of internal ROS is present in the irradiated lens at several depths. D : The control animal’s lens shows a very small amount of ROS near the lens surface. The dotted lines were placed on the lens sections to demarcate the position of the lens surface.

Article Snippet: Briefly, a computer-assisted Zeiss 2-photon LSCM (model 510 NLO, Carl Ziess, MicroImaging Inc., Thornwood, NY) was used on whole lenses immediately after extraction and subsequent 60 min immersion in MEM media containing 10 uM Hoechst 33342 dye for DNA detection and quantitation and 25 uM dihydrorhodamine (DHR) for ROS detection and quantitation.

Techniques: Irradiation